Description
The N-terminal elongation of the widely used MMP FRET substrate Mca-PLGL-Dpa-AR-NH₂ with a Lys yields a fluorogenic peptide with improved substrate properties. As compared to Mca-PLGL-Dpa-AR-NH₂, the specificity constant (kcat/Km) of the broad-spectrum MMP-substrate Mca-KPLGL-Dpa-AR-NH₂ for collagenases (MMP-1, MMP-8, MMP-13) and MMP-14 is increased two- to ninefold and threefold, respectively, while those for gelatinases and matrilysin remain equally high.
